enzymatic dna processing gold nanoparticles

enzymatic dna processing gold nanoparticles

Gold nanoparticle-based gene delivery: promises and

The effect of conjugation to gold nanoparticles on the ability of low molecular weight chitosan to transfer DNA vaccine. Biomaterials 2008, 29, 111–117. [62] Bhattarai SR, Remant Bahadur KC, Aryal S, Bhattarai N, Kim SY, Keun H, Hwang PH, Kim HY. Hydrophobically modified chitosan/gold nanoparticles for DNA delivery. Nanopart. Res. 2008, 10

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Application of DNA functionalized gold nanoparticles

Gold nanoparticles have been widely applied to different natural sciences due to its unique optical and electrical properties. It is easy to control the size and shape of gold nanoparticles to adjust the absorption peak of gold nanoparticles and change the optical and electrical properties. When gold nanoparticles are functionalized with DNA, they are endowed with the recognition ability and

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Enzyme-induced in vivo assembly of gold nanoparticles for

Dec 01, 2019 · Enzyme-conjugated gold nanoparticles for combined enzyme and photothermal therapy of colon cancer cells Colloids and Surfaces A: Physicochemical and Engineering Aspects, Volume 572, 2019, pp. 333-344 Download PDF View details

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DNAzymes, gold nanoparticles and disease detection: an

Apr 23, 2013 · Gold nanoparticles are tiny spherical particles made out of gold atoms with sizes on nanometre scale. This is around 1,000 times smaller than the thickness of human hair.

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HIGH SENSITIVITY DNA DETECTION USING GOLD

HIGH SENSITIVITY DNA DETECTION USING GOLD NANOPARTICLES AND CONDUCTING POLYMERS Author Elaine Spain B.Sc. (Hons.) Enzyme linked immuno sorbent assay EPR Electron paramagnetic resonance ES Emeraldine salt Et 2 O Diethyl ether 1.9.1 Basic features of the electrochemical process.. 54 1.9.2. Cyclic voltammetry .. 54 1.9.2.1 CV of

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Simple method for visual detection of ... - Gold Bulletin

Nov 20, 2015 · Hutter E, Maysinger D (2013) Gold-nanoparticle-based biosensors for detection of enzyme activity. Trends Pharmacol Sci 34:497–507. doi: 10.1016/j.tips.2013.07.002 Article Google Scholar

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Enzymatic Disassembly of DNA-Gold Nanostructures, Small

DNA cleavage by restriction enzymes is known to be highly specific for the recognition sequence. 22 We have shown previously that this specificity is maintained in the context of DNA complexed with nanoparticles, in experiments where DNA cohesive ends are revealed on particles by restriction enzyme action, and can then be covalently ligated to

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Gold nanoparticle DNA damage in radiotherapy: A Monte

May 17, 2016 · Chithrani D (2010) Intracellular uptake, transport, and processing of gold nanoparticles. Mol Membr Biol 27: 299–311. doi: 10.3109/09687688.2010.507787 [38] Yao X, Huang C, Chen X, et al. (2015) Chemical radiosensitivity of DNA induced by gold nanoparticles. J Biomed Nanotechnol 11: 478–485. doi: 10.1166/jbn.2015.1922

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A centrifugation-assisted visual detection of SNP in

DNA, which relies on repeated thermal cycles, and typically takes more than 2-14 days for a readout. The instrument-intensive and time-consuming process hampers the point-of-care (POC) assay application. Gold nanoparticles (AuNPs) based DNA colorimetric assay has been implemented as POC detection without sophisticated

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Enzymatic Formation of Gold Nanoparticles Using

When fungus Phanerochaete chrysosporium was challenged with gold ions under ambient aqueous conditions gold nanoparticles were formed within 90 minutes. Controlling experimental conditions like the age of fungus, incubation temperature and different concentration of gold chloride solution had drastic effect on the morphology of the nanoparticles formed.

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Nanoparticles based DNA conjugates for detection of

Jan 27, 2016 · Pioneer studies regarding the modification of gold NPs with oligonucleotides were reported way back in 1996 (Alivastos et al. 1996; Mirkin et al. 1996, as cited in [ 12 ]) and a number of studies have been reported since then concerning gold NP–oligonucleotide conjugate systems aimed at detection of complementary DNA sequences [ 13 – 16 ].

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Single Gold Nanoparticles as Real‐Time Optical Probes for the

Single Gold Nanoparticles as Real‐Time Optical Probes for the Detection of NADH‐Dependent Intracellular Metabolic Enzymatic Pathways † Lei Zhang Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237 (P. R. China), Fax: (+86) 21‐6425‐0032

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Gel Electrophoresis of Gold-DNA Nanoconjugates

Gold-DNA conjugates were investigated in detail by a comprehensive gel electrophoresis study based on 1200 gels. A controlled number of single-stranded DNA of different length was attached specifically via thiol-Au bonds to phosphine-stabilized colloidal gold nanoparticles. Alternatively, the surface of the gold particles was saturated with single stranded DNA of different length either

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Site-Specific Ligation of DNA-Modified Gold Nanoparticles

a) Enzymatic activation followed by ligation of DNA-modified gold nanoparticles. Gold nanoparticles, either small or large ( 15 and 60-80 nm), are symbolized by red and purple spheres, respec- tively, and DNA strands by blue and red bars.

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PDF Endonuclease Controlled Aggregation of Gold Nanoparticles

RNAse H, gold nanoparticles, aggregation, DNA detection, endonuclease enzyme 1. Introduction The on-site and sensitive detection of pathogens is of critical importance to the prevention, surveillance and control of infectious diseases and their outbreak at the first onset (Rhode et al., 2015; RNAseH Cl-Na+ Na+ Na+ Na+ Na+ Na+ Na+ Na+ Cl-Cl

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Gold Nanoparticle - an overview | ScienceDirect Topics

Gold nanoparticles (GNPs) can be easily synthesized using various techniques, depending on the required shape and size (Pérez-Juste et al., 2005).Their surfaces can also be simply modified and attached with several molecules, such as antibody, protein, carbohydrate, and lipid (Di Pasqua et al., 2009; Pissuwan et al., 2007).According to the review by Dykman and Khlebtsov (2011), many shapes of

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Gold nanoparticles speed up discovery of the genetic

Oct 24, 2011 · Oct 24, 2011: Gold nanoparticles speed up discovery of the genetic patterns responsible for disease (Nanowerk News) Many illnesses are now being linked to particular patterns of mutations in an individual's DNA.However, the search for these patterns, known as haplotypes, is generally a slow and painstaking process.

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Making More Sensitive DNA Sensors Using Gold Nanoparticles

This study focuses on the use of gold nanoparticles with DNA-based signal amplification as a detection method for low concentrations of DNA bio-markers. Biotechnology is a rapidly evolving field with primarily medical applications. Early detection is a challenging process for some cancers and other diseases yet is so critical to successful treatment. Increasingly sensitive detection techniques

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PDF Enzymatic Ligation Creates Discrete Multi-Nanoparticle

use an enzymatic exponential amplification method is explored as a means to amplify nanoparticle- DNA building block products. Enzymatic Ligation Analyzed by Electrophoresis. Gold nanoparticles functionalized with a single strand of thiolated DNA were isolated using an AE-HPLC protocol previously described. 17. Enzymatic ligation using the T4

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Precise Control of Gold Nanoparticles on DNA Origami for

Owing to their capacity for accurate structural control and complex programmability, DNA molecules have been extensively studied in relation to the construction of nanodevices. However, the existing logic gate sections based on DNA self-assembly were independent of each other, which hampered the development of large-scale integrated DNA circuits.

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Enhancing the efficiency of a PCR using gold nanoparticles

In Figure 6A, YEA DNA polymerase was used to react with 1.0 × 10 7 copies of DNA, the C T values with and without Au nanoparticles are 19.1 and 20.5, respectively. For the PCR with 1.0 × 10 6 copies of DNA, the C T values with and without Au nanoparticles are 21.9 and 26.3, respectively.

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Single Gold Nanoparticles as Real‐Time Optical Probes for

Single Gold Nanoparticles as Real‐Time Optical Probes for the Detection of NADH‐Dependent Intracellular Metabolic Enzymatic Pathways † Lei Zhang Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237 (P. R. China), Fax: (+86) 21‐6425‐0032

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Dithiothreitol-Regulated Coverage of Oligonucleotide

DNA-modified gold nanoparticles (AuNPs) are useful signal-reporters for detecting diverse molecules through various hybridization- and enzyme-based assays. However, their performance is heavily dependent on the probe DNA surface coverage, which can influence both target binding and enzymatic processing of the bound probes.

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Selective Enhancement of Nucleases by Polyvalent DNA

We demonstrate that polyvalent DNA-functionalized gold nanoparticles (DNA-Au NPs) selectively enhance ribonuclease H (RNase H) activity while inhibiting most biologically relevant nucleases. This combination of properties is particularly interesting in the context of gene regulation, since high RNase H activity results in rapid mRNA degradation and general nuclease inhibition results in high

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Green synthesis of gold nanoparticles and their anticancer

Jun 05, 2013 · Present investigation discloses the gold nanoparticles biosynthesizing capability of the flower of pharmacologically important tree Couroupita guianensis. Rapid, cost-effective, one-step process of synthesis has been achieved. Newly genre gold nanoparticles were characterized by involving UV–vis spectroscopy, FTIR, XRD, SEM, and TEM analysis.

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